The majority of mycotic infections are due to Candida species, out of which Candida albicans is being the most frequently isolated species. Eucalyptus is one of the very important and most widely planted genera across the world. It is a tall, evergreen tree, native to Australia and Tasmania, successfully introduced worldwide, now extensively planted in many other countries. Thus, the aim of the present study was to determine the antifungal activity of eucalyptus oil against clinical isolates of Candida spp. We have observed that, clinical isolates of Candida species were not inhibited in any of these dilutions of this oil. The eucalyptus oil does not have antifungal activity against Candida species. However, it is important to include more number of isolates and different dilutions to validate the results.