Biologically important polyphenol, (+)-catechin was isolated from Annona reticulata Linn fruit. The isolated (+)-Catechin was characterized by UV, IR, 1 H- NMR 13C- NMR and evaluation of its antioxidant and anti-cancer activities. High intake of fruits and vegetables has been correlated with a reduced risk of contracting different illnesses including cancer, diabetes, and cardiovascular diseases. This beneficial effect seems to be caused by phytochemicals with antioxidant properties as polyphenols, which have been proposed to be capable of controlling the oxidative stress caused by the overproduction of free radicals that can damage biomolecules such as carbohydrates, proteins, DNA and lipids. The (+) - Catechin showed significantly higher inhibition percentage at concentration 1000 µg/ml (62.42 ± 0.045) than MAR (45.55 ± 0.041) and positively correlated with total phenolic content. Cancer is a class of diseases in which group of cells division beyond the normal limits Invasion and metastasis. The profile of cell growth after treated with (+)-Catechin and MAR are found that both (+)-Catechin and MAR showed a significant reduction in the number of viable cells at the concentration higher than 1000 treatment with (+)-Catechin (87.40±1.2) was more than MAR (66.56±0.2) against MCF-7 cell lines.
