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Blood parasite constituent a major problem in goat breeding due to severe economic losses and lowered vitality which lead to a decrease of animal production and increase the susceptibility to bacterial and viral infections. The only common feature between these diseases is that they can all be transmitted by ticks. The objective of the present study was to determine the prevalence of Anaplasmosis in Iraq goats of Baghdad city and differentiation between Anaplasmaovis and Anaplasmamarginale by using PCR and PCR-RFLP. The results revealed that 55 out of 100 blood samples were positive for Anaplasmaspp. Infection. All positive samples were A. ovis as confirmed by restriction fragment length polymorphism (RFLP) method .Results showed that PCR-RFLP of the MSP4 gene aspecific method for the detection of A. ovisspecies in goats.