There are various health benefits of Tulasi, yellow camphor, ajwain, etc., that have been used in different ayurveda techniques. They help combat diseases, overcome variegated problems, weight loss and to prevail over various issues. This research studies the retention of anti-bacterial activity in six different water samples, which have been stored over a period of time. Tulasi, cardmom & yellow camphor sample, holy water, regular water stored in tupperware container, regular water stored in plastic container. oma/ajwain water are such samples. These have been subjected to bacterial growth studies by two techniques (colorimeter-turbidity and colony counting). The results obtained showed that ajwain water has been the most effective one as it had the least amount of growth of bacteria, owing to its immense medicinal properties; followed by traditional theertham of hindu temples and holy water obtained from a church.