Background: According to the World Health Organization, the efficacy of infection prevention and control protocols, as well as the provision of superior quality healthcare, is contingent upon health facilities adhering to appropriate sanitation standards and reducing the risk of pathogen exposure for both patients and healthcare workers. Infections that may lead to adverse pregnancy outcomes, sepsis, or even mortality among pregnant women and their neonates necessitate particular vigilance to avert. Throughout each phase of the sanitation service continuum—ranging from the provision of safe toilets and proper containment to the transportation, treatment, and ultimate disposal or intended use of waste—a comprehensive sanitation system is designed and executed to ensure the segregation of human excreta from human interaction. When sanitation safety is strategically planned, it facilitates the implementation of a holistic approach to address faecal management issues, encompassing everything from toilets to secure disposal or utilization. Healthcare-associated infections (HAIs) impact hundreds of millions of individuals globally on an annual basis. Despite being recognized as the most prevalent adverse occurrence in healthcare settings, the comprehensive global ramifications of HAIs remain ambiguous due to the difficulties inherent in acquiring reliable data. A principal objective of the WHO’s Infection Prevention and Control (IPC) team is to understand and assess the worldwide prevalence and impact of HAIs. This study endeavours to evaluate the degree of implementation of Sanitation and Hygiene practices within sub-district hospitals in the Gadag district and to ascertain opportunities for enhancement. Objectives: To assess the level of implementation of sanitation and hygiene guidelines in sub-district hospitals in Gadag District. Methods: A cross-sectional study was conducted in sub-district hospitals in Gadag district, and an universal sampling technique was used to choose the health centers. A kayakalp checklist 2021 was used to obtain the data from December 2023 to January 2024. Data were entered into Microsoft Excel, and results were expressed in frequency and percentages. Results: Total 4 sub-district hospitals were visited; the total score was 100 in that Mundaragi Sub-district hospital has 65/100 (65%), Shirahatti Sub-district hospital 67/100 (67%), Rona 70/100 (70%), and Naragund 63/100 (63%). Conclusion: The findings from the different sub-district hospitals show differences in how sanitation and hygiene regulations are applied. While some hospitals adhere to cleaning guidelines in important areas with great diligence, others need major improvements, especially when it comes to the cleanliness of auxiliary areas, restrooms, and the oversight of cleaning activities.
