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Consequence of the chemical mutagen Sodium azide has been studied on the vegetable crop Trigonella foenum-graecum L. Mutagenic treatments were given in the form of 6 Hrs pre-soaking in Sodium azide, after pre-soaking them in distilled water for 12 hrs. Various physiological parameters like proteins, carbohydrates were evaluated, along with chlorophyll content analysis. The level of amino acid proline was also assessed. It was found that treatment found to have significant inhibitory effect on seed germination. Strong enhancement in parameters like proteins and chlorophyll was recorded. In the plants treated with Sodium azide there was significant increase in the level of amino acid proline which is a stress induced amino acid. There was a considerable increase in amount of total carbohydtrates in plants with Sodium azide treatments