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Bacteriological and physico-chemical quality of Tapi River was assessed in order to determine seasonal and spatial variations. Samples collected from three different sampling sites along the Tapi River bank were analyzed for physico-chemical parameters viz., Temperature, pH, DO, BOD, Nitrate, Phosphate, Ammonia and Bacteriological parameters viz., Total Viable Count and presence of selected pathogens. Moderate temperature, alkaline pH, decreased DO and increased BOD and other nutrients as well as higher bacterial load at downstream sites were the common observations throughout the study period. Seasonal variations in all the parameters were observed. Water quality was found poor during summer comparatively. Frequent presence of potential pathogens at all three sites indicates the risk of infection and health issues and therefore it is a matter of concern.