Pesticides penetrate into aquatic ecosystem by several ways viz., drift, wash off and drain from agroecosystem or by conscious application in the water. Hoplobatrachus tigerinus live in freshwater and thus continuously get exposed to the contamination by contact, respiration and by contaminated food intake. We therefore hypothesized that these sub-lethal yet environmentally significant concentrations of imidacloprid has toxicological implications on Hoplobatrachus tigerinus. The validation of the study was to assess glycogen and proteins analysis in vital organs of Hoplobatrachus tigerinus exposed to Imidacloprid, after 24, 48, 96 h, 8, 15 and 30 days of exposure. The results obviously exemplify that exposure to sub-lethal concentration of imidacloprid (1/10th of 96 h LD50) was reflected in tissue concentrations of pesticide with significant alterations in the biochemical parameters glycogen and proteins. The total glycogen levels decreased on exposure to sub-lethal concentration of Imidacloprid. Among the various test tissues, higher glycogen content was observed in liver. This is due to the involvement of liver in glycogen synthesis and utilization. Maximum decrease of proteins was noticed in liver and muscle. The minimum decrease was almost equal in brain and kidney tissues. The depletion in the protein may be due to metabolic utilization of the ketoacids in gluconeogenesis pathway for the synthesis of glucose or may be due to directing the synthesis of proteins from free aminoacids. These results are in agreement with several studies reported in the literature. Risk assessment of pesticides in aquatic ecosystem achievable by the estimation of acute and chronic toxicity.
