The development of eco-friendly technologies in material synthesis is of considerable importance to expand their biological applications. A variety of green nanoparticles with well-defined chemical composition, size, and morphology have been synthesized by different methods and their applications in many technological areas have been explored. Efficient and green chemistry approaches has led to the use of microorganisms. Native Lactobacillus of Iraq derived from some local fermented products was used in this study. Total of 15 Lactobacillus isolates were isolated from local fermented food in Najaf city inoculated on de Man, Rogosa and Sharpe (MRS) agar. Plates were incubated for 24 hrs at 30 °C under anaerobic condition. Titanium nanoparticles had antibacterial activity against indicator strain (Pseudomonas aeruginosa). The efficient isolate (MT14) of Lactobacillus was selected for titanium nanoparticles formation and identification. Titanium nanoparticles formation by Lactobacillus in some aspects was observed during the study. Characterization of nanoparticles was done by SEM /EDS analysis. Scanning electron Microscope revealed the formation of spherical nanoparticles with size ranging between 50-90nm. Energy dispersive spectroscopy (EDS) was done using SEM instrument equipped with Bruker EDS attachment showed the optical absorption peak. The efficient isolate (MT14) of Lactobacillus was identified by morphological and biochemical characteristics and confirmed by using species specific primers for the polymerase chain reaction (PCR) that enable specific detection of Lactobacillus rhamnosus. The primers amplify a 122 bp DNA sequence of the genus Lactobacillus rhamnosus.
