Like other halogenated alkanes carbon tetrachloride or tetrachloromethane (CCl4 ) induces hepatorenal injury in both acute and chronic cases in rats. Rats were sequentially administered with a dose of 115 mg/kg of body weight /day and 230 mg/kg of body weight /day respectively for 7 days and also an acute dose of 2000 mg/kg of body weight was also given for mortality, wellness parameter study. Several oxidative stress markers, biochemical parameters and liver and renal functions tests were measured. The present study was aimed to determine LD50 of CCl4 by subcutaneous application in male rats as per the guidelines of Organization for Economic Cooperation and Development (OECD). Our results suggested that the CCl4 induction causes hepatorenal toxicity by generation of liver fibrosis in experimental animals, at a concentration of 230 mg/kg bw/day in a time period of 7 days subcutaneous administration in male rats.
