Freshwater ecosystems throughout the world play very crucial role in ichthyofaunal diversity as well as fishery potential.North-east India is considered a hotspot of ichthyofaunal resources. The Palla river flows through the Baksa and Barpeta districts of Assam. It is a tributary of the Chaulkhowa river, which merges with the Brahmaputra River. In the present study, a total of 56 species of fish have been identified under 43 genera, 8 orders and 19 families from the four sampling sites of the Palla river, Assam, India (Table 1). The identified species of fish belongs to the following families: Notopteridae (2 species), Ophichtidae (1 species), Clupeidae (1 species), Cyprinidae (15 species), Botidae (2 species), Cobitidae (4 species), Nemacheilidae (2 species), Sisoridae (5 species), Siluridae (4 species), Chacidae (1 species), Clariidae (1 species), Heteropneustidae (1 species), Schilbeidae (1 species), Bagridae (5 species), Belonidae (1 species), Ambassidae (1 species), Badidae (2 species), Badidae (4 genera), Nandidae (1 species), Channidae (4 species) and Tetraodontidae (1 species) and syngnathidae (1 species).