This investigation was carried out to study the effect of some Plant Growth Regulators and Coconut Water on callus Induction using vegetative parts such as Nodal explants in Tinospora cordifolia (Wild.) Miers family: Tinospora cordifolia (Wild) an important medicinal plant. Callus induction was carried out using different concentrations of Auxins (2, 4-D, IAA, NAA), (1.0–7.0 mg/L) and cytokinins (BAP and kinetin), (2mg/L) alone and in combinations with 5-15% (v/v) Coconut Water. Among the different concentrations of Coconut Water tried, MS medium with NAA+ Kinetin (6.0+2.0 mg/L) along with 5% (v/v) Coconut Water and MS medium with 2,4-D + Kinetin (6.0+2.0 mg/L) along with 10% (v/v) Coconut Water showed best callus response (96.6%) in nodal explants. Whereas nodal explants showed the next best callusing response (93.33%) with 10% (v/v) Coconut Water on MS medium with NAA + BAP (4.0+2.0mg/L) and (7.0+2.0mg/L) respectively. Similarly with IAA + kinetin (7.0+2.0mg/L) + 10% (v/v) Coconut Water, response was 96.6%. The Sub-culturing time required was found to be longer in medium containing Coconut Water.
