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The objective of this study was to determine the antimicrobial and antibiofilm properties of the organic extracts of Pityrocarpa moniliformis obtained by different techniques of extraction against several microorganisms. Antimicrobial and antibiofilm activities were determined using the serial microdilution method in 96-well culture plates and the crystal violet method, respectively. Most extracts showed antimicrobial activity in all evaluated microorganisms and Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were achieved for some extracts at the concentration of 2 mg/mL. In addition, the methanolic extracts showed antibiofilm activity against most of the tested microorganisms. Therefore, the extracts of P. moniliformis proved to be efficient for the activities tested, demonstrating their potential commercial application.