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Aim: The aim of this in vitro study is to compare the antibacterial efficacy of Octenidine Dihydrochloride and Calcium hydroxide, Triple Antibiotic Paste, Turmeric and Calcium hydroxide as anintracanal medicament against Enterococcus faecalis. Material & Method: The samples were divided into 3 groups. Group A: Octenidinedihydrochloride+ Calcium hydroxide, Group B: Triple Antibiotic Paste, Group C : Turmeric +Calcium hydroxide. Enterococcus faecalis are grown in brain heart infusion broth (BHI). Three wells 8mm diameter were punched in each media plate and filled with respective groups of medicaments. After incubation period of 24 hr, at 37º C, inhibition zones are measured with vernier caliper. Results: The Triple antibiotic paste showed highest zone of inhibition than other groups. Conclusion: Under the limitation of this study, all the materials showed antibacterial activity against Enterococcus faecalis but Group B(Triple Antibiotic Paste) showed best efficacy against E. faecalis.