Background: Diabetes mellitus is a group of metabolic diseases characterized by elevated blood glucose concentration (hyperglycemia) resulting from either defect in insulin secretion by the pancreas, insulin action, or both. According to World Health Organisation, over 422 million people have diabetes in the world while 24 million people in Africa have the disease but this prevalence is expected to rise by 129% by 2045. Materials and Methods: Forty (40) samples were selected for testing and analysis. The glucose level of each patient was assayed using both the glucometer and glucose oxidase method with the samples run in duplicates and measured simultaneously.The results were compared using means, standard deviation, median (range), and correlation. Results: The means were 4.7 mmol/l for the reference method and 4.65 mmol/l for the On Call Plus. The results of the study were found to be comparable with a correlation coefficient of 0.9553 and an accuracy of 99%. The bias was 0.2350 and p-value < 0.0001. Conclusion:The study found a strong correlation coefficient of 0.9553 between glucose measurements with On Call Plus and the laboratory reference method, an accuracy of 99% between the two modes of testing, and an acceptable precision of less than 10% observed. The results are statistically significant with a p value of <0.0001. It is concluded that a comparability of results of On Call Plus glucometer to those of the ILab 300 plus could be obtained if the sources of error are minimized.
