The objective of this research was the elaboration and validation of an instrument to measure the Quality of Work Life (QWL), with the purpose of verifying the usefulness and validity of the instrument to be used in a research project called the Quality of Work Life in public, private and educational organizations. The designed instrument had 70 items that identified the perception of the workers with respect to 15 dimensions, responses were included with a Likert-type scale, 82 surveys were applied randomly in different organizations during 2017.The results of the validation were the following: content through the Panel of Experts; reliability with Conbrach alpha 0.952; Kendall coefficient of concordance 0.394; the validity of the construct with the test KMO 0.870 and the sphericity of Bartlett 0.000; A correlation analysis was performed and found relations in all the variables. The contribution of this article is a QWL instrument that can be applied to all organizations, since it is regularly associated with burnout and stress in workers in the health sector. It is concluded that the instrument meets the optimal validity to be used in future organizational research.