Azima tetracantha Lam. (Salvadoraceae) is widely used in folklore herbal medicine practices in the villages of Avidanallavijayapuram Orathanadu Taluk Thanjavur District. The plantpossess antiinflammatory, antiperiodic, analgesic and wound healing properties. The medicinal plant Azima tetracantha lam leaf extract was analysed for the determination of phytochemicals and antimicrobial activity. The phytochemicals evaluation showed the presence of alkaloids, terpenoids, phenols, tannins, saponins, quinines, protein and steroid in various solvents like ethyl acetate, chloroform, methanol and aqueous extracts. The In-vitro antimicrobial activity was studied against human pathogens of gram positive strains such as Bacillus subtillis, and Staphylococcus aureus, and gram negative strains Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and three fungal strains: Aspergillus niger, A. fumigatus and A. flavus using disc diffusion method. The effect of these extracts were compared with standard drugs penicillin and streptomycin. The phytochemical screening of the extracts revealed the presence of secondary metabolites like alkaloids, flavonoids, tannins, triterpenoids, saponins etc.
