The current study included determining some Heavy metals in water on Al-Dalmaj marsh south of Iraq by use scanning electron microscopycoupled with energy dispersive spectrometer(SEM-EDS) .Samples were collected in two seasons winter and summer for water. The chemical studied parameter such pH, and heavy metals concentrations , which indicate polluted as it . The results showed increased of heavy metal concentrations comparative with WHO concentrations whereas: Mn:4.2584-3.334 , Cd : 0.276226-0.16226 , Ni : 1.0349- 0.5328 , Pb : 1.2765-0.8305 , Co : 1.0183-0.6176 , Cu : 1.9787-1.7746 , Zn : 11.789-9.2054 , Hg : 0.6789-0.2434 µg/ml, respectively in two seasons Sumer and winter. The range of pH was 7.9-8.8 during the winter, while in summer it was slightly acidic to alkaline (6.6-7.4).The results of enumerate bacteria of plate counts on media MacConkey agar and EMB agar gave much higher growth to various species on EMB agar media for 1,2,4 and 5 from the sites, comparative between MacConkey ager media .