Development And Validation Of Chromatographic Method For Estimation Of Canagliflozin In Api And Tablet Dosage Form

A simple, specific, precise and accurate chromatographic method for estimation of canagliflozin in API and tablet dosage form was developed by C18 column having 250 mm length, 4.6 mm internal diameter, 5μ particle size. Peak was observed in mobile phase consist of Methanol: Acetonitrile: 0.1 % Ammonium Acetate in the proportion of 40:40:20 v/v/v. The flow rate was 1ml/min. The estimation was carried out at 290 nm. The retention time was found to be 4.1 minute for Canagliflozin. Linearity was found in range of 100-300 µg/ml. The method was validated as per ICH guideline Q2R1. All validation parameters were found to be within accepted range specified in ICH guideline Q2R1.