An accurate, precise, robust, sensitive and simple Reverse-Phase HPLC (RP-HPLC) isocratic method was developed and validated for analysis of Febuxostat Standard. The chromatographic conditions of Febuxostat (pure) standard was performed on Kanak C-18 (250 x 4.6mm, 5.0μm) column that was performed at 25°C temperature when10μl volume was injected into the system.The mobile phase used was Phosphate buffer (pH 2.5): Methanol, 20: 80 (v/v) at flow rate of 1.0ml/min and detection was carried out at 316nm. The retention time obtained was 5.6min (± 0.5min) that was found to be linear in the range of 10-50 ppm. Limit of Detection (LOD) and Limit of Quantification (LOQ) obtained was 0.024 and 0.078ppm respectively. The %RSD for reproducibility, precision and robustness parameters were within the specified limits according to ICH guidelines. The analytical investigation of Febuxostat drug on Inertsil column showed peak with wide fronting and tailing as compared to the peak shape obtained on KanakTM column.
