Microbial pathogens have a survival capability in a variety of environmental conditions and adhere themselves with different surfaces, thus forms a matrix like structure called biofilm. These biofilms provide unconditional environment for the pathogenic microbes that have barrier to the migration of drugs, thus facilitate the microbes to resist towards various antimicrobials. Thus, this study was designed to analyze comparatively about the biofilm formation among the antimicrobial resistant bacterial strains by test tube adhesion method using seven different stains where crystal violet act as a control. The drug resistant bacterial pathogens were allowed for biofilm production and determine it on the surface of test tubes using crystal violet, methylene blue, nigrosin, safranin, Gram’s iodine, leishman, giemsa stains. The results revealed that the observation and recording of biofilm deposit on the test tubes showed better and maximum while using crystal violet followed by methylene blue and safranin. The test bacterial pathogens like Klebsiella sp, Proteus vulgaris and S. aureus showed maximum adhesion and adsorption of the stains.
