The aim of this study was to identify the rock lobster P.homarus with the help of DNA Barcoding. Molecular taxonomy of P.homarus was performed using Cytochrome Oxidase I, Histone H3 and 18S rRNA gene loci. Isolation of genomic DNA was performed with the help of CTAB buffer and later with the help of Polymerase Chain Reaction COI, Histone H3 and 18S rRNA genes were amplified. Sanger’s method of DNA sequencing was used, and sequence chromatogram was obtained for all the three gene loci. The DNA sequences of the respective genes of P.homarus were analyzed using Finch TV 1.4 and the similarity of sequences was understood using BLAST (Basic Local Alignment Search Tool). FASTA sequences of P.homarus were compared with the FASTA sequences of other related lobster species using Multalin tool and Clustal Omega. The evolutionary relationship between the P.homarus and other lobster species was studied with the help of Multialin tool. Evaluation of Phylogenetic relationships between P.homarus and other lobsters from same family was performed using Clustal Omega. Therefore, in this research study we conclude that COI, Histone H3 and 18S rRNA can be used to identify various lobster species.
