Caesalpinia sappan L belongs to family Caesalpiniaceae, a plant with high medicinal values. The present study evaluated the effect of different combinations of plant growth regulators on callus induction using different vegetative parts in different plant tissue culture media. Maximum callus induction response was observed in nodal explants (93.3%) in MS medium supplemented with NAA (2mg/L), BAP (2mg/L) whereas in medium supplemented with IAA (1mg/L) and KIN (2mg/L) the response was 78%. The callus response was 41% in MS medium supplemented with 2, 4-D (2mg/L) and Kinetin (0.5mg/L) and the callus response was very less(26%) in MS medium with 2,4- D(0.5mg/L). The callus were white compact or friable in nature. A combination of Auxins and cytokinins was found to be effective in induction of callus in Caesalpinia sappan L, when compared to using individual plant growth regulators. In this study callus induction and proliferation protocol has been established.
