Rose (Rosa hybrida L. cv. bush rose) micropropagules were grown on salicylic acid (SA) supplemented MS medium with the objectives to study its effect on growth and multiplication under in vitro conditions. Salicylic acid was added in the MS medium prior to autoclaving as well as after autoclaving, through filter sterilization. Change in the mode of addition of SA did not make any significant changes in growth as well as biochemical parameters. Low concentration of SA (5 mg l1 ) was proved to be good in terms of higher accumulation of biomass and growth while higher concentration exerted subdued effect. Similarly, various biomolecules viz. carbohydrates, proteins, phenols and chlorophylls also shown significant enhancement in the micropropagules grown on the medium supplemented with low concentration (5 mg l-1 ) of SA. It was concluded that SA at low concentration can be used as potential growth regulator in rose micropropagation. Further, it was noted that mode of application of SA does not make any significant change in growth pattern in rose micropropagules.
