Aim: This in-vivo study was conducted to determine whether there is any effect of fluoride incorporated composite in the reduction of enamel colour change when compared with a conventional composite resin after debonding of orthodontic brackets, exposed to the same intraoral environment when subjected to orthodontic treatment. Materials and methods: Total sample of 20 patients comprising of 80 teeth was divided into two groups consisting of 40 teeth each. Group I was bonded with fluoridated composite (study group) and Group II was bonded with conventional composite (control group) using split mouth design. Debonding was done after three months. Enamel colour was evaluated by spectrophotometer at baseline and after debonding, and the corresponding colour differences ΔE and ΔL were calculated. Results: Both groups exhibit colour difference since all the ΔE values were more than 1 unit. However ΔE values did not exceed 3.7 units indicating the colour change is not clinically perceivable. There is a significant difference (P<0.05) in the ΔL values between the experimental group and control group indicating fluoride does have effect over the enamel colour change. When all the four quadrants were compared, the enamel colour change seen in the fourth quadrant is significantly higher than the other three quadrants. Conclusion: There is significant enamel colour change caused due to orthodontic treatment. Use of fluoridated adhesive has significantly reduced the enamel colour change caused due to orthodontic treatment.