An in vitro anther culture method was standardized for producing double haploid plants in 10 F1 genotypes of Chilli pepper (Capsicum annuum L.). This procedure was studied to optimize and study the effect of culture media and each genotype for androgenic response. Androgenesis varied depending upon the genotype and media. Ten genotypes were evaluated in 3 variations of media concentrations. All the genotypes showed differential expression in different media compositions. Direct embryogenesis for different genotypes varied from 4.1% to 38%, PH 53 showed the highest and lowest rate of embryogenesis was observed in PH 17 genotype. It was observed that MS medium with 0.1mg/L Kinetin and 0.5mg/L 2, 4 Dichlorophenoxyacetic acid (2,4-D) gave higher embryo frequency in PH 53 and lowest in PH 51 genotype. MS medium with 3mg/L 1-Napthalene acetic acid (NAA) and 1mg/L 6-Benzyl amino purine (BAP) gave higher embryo frequency in PH 59 lowest in PH 17 genotype where as MS medium with 0.5mg/L NAA, 1mg/L Kinetin and 0.5mg/L BAP gave higher embryo frequency in PH 57 followed by PH 43 genotype.
