Impact of pre-emergence herbicide Butachlor 50% EC and post-emergence herbicide 2, 4-D 80% WP was evaluated to freshwater fish, Labeo rohita. Chronically fish were exposed for 21 days to 0.066 mg/L Butachlor 50% EC, 100 mg/L 2,4-D 80% WP and in combination of the exposed concentration. Significant increase in total protein was observed in the gill, liver, kidney and muscle tissues exposed to the combination of herbicides and similar change was observed in the gill, liver, kidney and muscle in groups of fish exposed to Butachlor 50% EC. Whereas, in 2,4-D 80% WP significant increase was observed in liver tissue. Increase in aspartate transaminase and alanine transaminase in gill, liver, kidney and muscle tissues were observed in the fish exposed to the 2, 4-D 80% WP + Butachlor 50% EC. Elevation and inhibition in enzymes might be due to stress, disruption of enzyme systems and tissue damage.
