An Efficient In Vitro Regeneration Of Multiple Shoots From Leaf Explant Of Passiflora Caerulea L. An Important Medicinal Plant

An efficient in vitro protocol was established to produce multiple shoots from leaf explants of Passiflora caerulea L. on Murashige and skoog (MS) basal medium complemented with auxins and Cytokinins. Different concentrations of Benzyl amino purine (1.0 to 4.0 mg l-1 ) were assessed for multiple shoot production. Large number of (more than 100) multiple shoots per explants were induced on medium containing 3 mg l-1 BAP. Rooting was achieved on half or full strength MS medium with 0.5-1.0 mg l-1 IBA (Indole-3-butyric acid) in 28 days. Regenerated plantlets acclimatized and transferred to soil showed normal morphological characteristics. Survival of transplants was 95-100 % under green house condition.