Estimation Of Isoquercitrin By Hptlc Method In Hygrophila Salicifolia, (Acanthaceae)

Traditionally Hygrophila salicifolia (Acanthaceae) is reported to possess anti-inflammatory and diuretic properties. The objective of the current study was to develop HPTLC method for quantification of Isoquercitrin from the methanolic extract of Hygrophila salicifolia which can be used in quality control of the crude drug. Chromatographic analysis of methanolic extract of the whole herb gave typical fingerprints, where, Isoquercitrin, a standard reference marker, resolved at Rf 0.76 under TLC condition. One dark black colour band at Rf 0.76 under the TLC conditions was characteristic of the raw drug. Isoquercitrin was quantified to be 229.801ug/ml using the HPTLC method.