The aim of the present study was to evaluate the pesticide residues in human blood samples of volunteers related to Jaipur, Bhilwara and Ajmer districts of Rajasthan. The volunteers were categorized into two main groups on the basis of gender and age. Group I was subcategorised as male and female and Group II was subcategorised according to their age: 1 to 3, 20 to 30 and 50 to 70 yrs. Qualitative analysis of pesticide residues in blood samples were carried out by using thin layer chromatography. 33% samples were found to be positive regarding presence of pesticide residues in their respective blood samples out of the total samples analysed by thin layer chromatography. In the present study qualitative and quantitative estimation of pesticide residues were carried out by gas chromatography-Mass spectrometry. The blood samples of 15 volunteers were analysed for pesticide residues using GC-Mass spectrometry. 29 % of female volunteers were found positive for presence of pesticide residues in their blood samples whereas 62 % of samples of male volunteers were found positive for the presence of pesticide residues in the blood samples analysed by GC-Mass spectrometry. Out of 5 blood samples of infants analysed for pesticides residues, Deltamehtrin was detected in one of the sample (0.18mg / ml). 40% of the male volunteers belonging to age between 30 to 35 years were found to contain residues of chlorpyrifos in their blood samples with concentrations range of 0.002836- 0.241210 µg /ml. Cypermethrin was also detected in blood samples of 35% of male volunteers with the concentration range of 0.344841 - 0.543612 µg /ml .The residues of methyl carbamate was detected in 20% of the female volunteers of age belonging to 50-55 years with the concentration range of 0.003345 - 0.005632 µg/ml. The quantity of pesticide residues detected in some blood samples of agro-professionals were found to be at the alarming level.
