The current study was conducted on water quality of Imphal River, Manipur. The aim of the study is to analyse physico-chemical properties of water of Imphal River. Imphal River is one of the main sources of water for the people of Manipur. The study was conducted from Oct. 2014 to Dec. 2015. The water samples were analysed using APHA standard methods. The physico-chemical parameters like water temperature, PH, dissolved oxygen, Free Carbondioxide, total alkalinity, turbidity, total hardness, calcium, magnesium, sodium, potassium and chloride were evaluated. The value of water temperature recorded between 16.5o C- 27.9o C, pH ranged between6.3-7.9, dissolved oxygen between 5.8-19.3 mg/l, Free CO2 between 0.5-7 mg/l, alkalinity ranged 10-84 mg/l, turbidity between 0-480 mg/l, total hardness between 32-52 mg/l, calcium between 8.46-16 mg/l, magnesium between 2.73-9.95 mg/l, sodium between 3.46-6.02 mg/l, potassium between 1-3.32 mg/l. The river water is contaminated in the city areas due to human activities and there is a need to monitor regularly to conserve the water system. So, the State Government should make proper policy programme to prevent further degradation of the river since many people depend on the river for drinking and other livelihood like fishery activities.