Nanobiotechnology is modestly too vast and too rapidly changing to cover exhaustively. Nanoparticles which play important role in the drug delivery systems and for treatment. This is because of its potential of possessing large surface areas and essentially no inner mass, i.e., their surface-to-mass ratio is extremely high. Recently researchers have found a new method for synthesis of silver nanoparticles (AgNPs) called green synthesis, from both plant and microorganisms such as bacteria, fungi and actinomycetes. The aim of the present study was, biosynthesis of silver nanoparticles (AgNPs) using Lactobacillus sp. which is one of the group of microorganisms, used as probiotics, which are safe and can be used in pharmacology industries. Lactobacillus was cultured in nutrient broth and incubated at 37°C for 24 h. Then they were centrifuged and supernatant was saved. To the supernatant, silver nitrate was added to and incubated for 28h at 35-37 °C. The formation of AgNPs was monitored by colour change and confirmed by the different characterizations of UV-Spec, FTIR, SEM and EDS. This research article confirmed the beneficial uses of extracellular biosynthesis and characterization of silver nanoparticles from Lactobacillus acidophilus for the treatment of gastro intestinal treatment.
