Phyto Chemistry is a fast growing study of Phyto Chemicals which are chemicals derived from plants. The phyto chemical include the flavanoids terpenoids, lignans, polyphenotic etc. In the present study lemon grass was taken for many pharmacological activities. Lemon grass belongs to poacea family, lemon grass were collected, dried, powdered and extracted with water and chloroform. and Extraction done by soxhlet apparatus. Separation of lemon grass extracts were done by column chromatography method. Phytochemical screening tests were carried out and analysis showed that it contain alkaloids, flavanoids, tannins and phenols. The constituted were estimated using electrometric method. Alkaloids estimated by “SINGHMBTH” method flavanoids estimated by ZHISHEN Alcl3 Method. Phenols were estimated by FOLIN – CIOCALTEAU method and finally tannins by Prussian blue method. Concentration of the extracts were known and amount of phyto constituents were calculated. Pharmacological evaluation of extracts done by Anti-Oxidant method Namely Nitric Oxide, hydroxyl free radical scavenging activity. The Anti Bacterial activity were detected by disc diffusion method the dispenser method using species Ecoli and staphylococcus aureas. The result of analysis establish the pharmacological potential of lemon grass.
