Water is an essential part of everyday life. But industrial revolution coupled with population explosion create stress on environment and introduce harmful chemicals and various pesticides in fresh water resources. Those pesticides and chemicals accumulates in the aquatic organisms and have detrimental effects on metabolism and physiology on non-target organisms in fresh water bodies. Soit was decided to assess the toxicity and biochemical constituents in mucus of the experimental fish Channa gachua (F. Hamilton)exposed to dichlorvos.The fishes were exposed to LC0 (5 ppm) and LC50 (12.5 ppm) concentrations of dichlorvos for 96 hrs. (acute). After exposure to dichlorvos the glycogen was increased,lipid was decreased and fluctuations in protein was observed in the body mucus of fish.The pesticide intoxication might disturb the normal functioning of the cell with the alterations in the biochemical moieties in the exposed fish.