Nanotechnology has a wide range of applications in field of medicines. Nanoparticles are the fundamental blocks of nanotechnology. Among all the noble nanoparticles, silver nanoparticles are widely studied due to their unique properties. For the synthesis of silver nanoparticle plant extract is used as it easily available and capable of producing nanoparticles in large scale. The silver nanoparticles are formed by mixing the plant extract in silver nitrate (AgNO3). The present study was done in Clitoria ternatea belonging to family Fabaceae. The synthesized nanoparticles from the plant extract were confirmed by UV-Vis spectrophotometer, FTIR and XRD. Color changed from yellow to brown color indicated the presence of silver nanoparticles in the plant extracts. The result showed that Klebsiella pneumoniae shows higher zone of inhibition than the others, in which silver nanoparticles synthesized by stem part shows higher activity.