Fresh water aquaculture in India is mainly based on Indian major carps (Labeo rohita, Catla catla, mrigala) with an annual production of 1.66 million tons. And exotic carps, which together contribute to over 87% of total aquaculture production of 1.93 million tons (FAO 1999).The cattle urine are beneficial to the filter feeding and omnivores fishes. Urine consisting of water (95%), Urea (2.5%), and (2.5%) of minerals, salts, hormones and enzymes which have been scientifically proven as safe medication. So the present investigation growth and survival parameters were carried out in detail about the different breeds of fresh cow urine activity. Labeo rohita fingerlings treated with 0.1% concentration of three breeds of cow urine. The growth parameters were analyzed and compared to the control groups. Water quality variables such as salinity, EC, TDS, Turbidity etc., the results of this study prove that cow urine as ecofriendly bio enhancer.