The uses of medicinal plants in prevention and treatment of diseases attracting scientist’s attention worldwide. It is necessary to bring vital healthcare and the better substitute for various diseases. In the present study extracts of Toddalia asiatica root, stem and leaf was assessed for their in vitro anti-oxidant, Anti-lipid peroxidation activity, lipoxygenase inhibition by spectrophotometerically and inhibition of Human plural fluid phospholipase A2 (HPF-PLA2 ) by egg yolk plate method. Among extracts, ethanolic root extracts of T. asiatica and aqueous root extracts of T. asiatica exhibits grate phytochemicals content. The ethanolic extract of T. asiatica exhibit great DPPH free radical scavenging activity (activity ranges between 71.72 to 82.03%) and anti-lipid peroxidation (76.74 to 86.25%). The ethanolic and aqueous root extracts of T. asiatica shows 90% and 72.36% of HPF-PLA2 inhibition at 50μg concentration. Further, ethanolic extract of T. asiatica was inhibited LOX enzymes in concentration dependent manner and the IC50 values rages from 36.04 to 55.2±0.05 μg/μl for the 5-LOX and 38.08 to 51.26±0.05 μg/μl for 15-LOX respectively. The study suggests that there is a strong correlation between anti-inflammatory activity and the phytochemical contents (might be flavonoids). The plant with further studies directed towards detection of bioactive molecules as potential lead candidates.
