Advance of molecular approach and biochemical studies, we identify elite biotypes of Terminialia arjuna which are grown in local area of Telangana region. Using five RAPD primers only one primer viz. OPP-3 produced unique DNA bands in 8 genotypes of T.arjuna, which could be used as differential marker for identification elite biotype in T. arjuna. We identified the elite biotype of Jakaram-FDA with unique band (2500, 2000 bp) and produce maximum protein content (369 ml/5gm), while this genotypes produced large size cocoon (19.27±0.9 gm). Minimum amount of protein was observed in EturnagaramFDA (206 ml/5gm) & produced small size cocoon (4.9±0.14 gm). Using UPGMA method dendrogram was constructed by OPP-3 primer showed that 10 genotypes of T. arjuna grouped into two main clusters. First cluster revealed 75% and second cluster revealed 65% genetic similarity. This method is useful to conserve elite biotype (high yield), which producing quantitative and qualitative cocoon, which are used in silk industry.
