The ability of a species to pass on its genes to future generations determines its survival. This study was undertaken to ascertain the effect of creatine monohydrate on the reproductive (fitness mating latency, copulation duration and fertility) of D. melanogaster. In the current study, we discovered that mating latency increased with increasing CrM concentrations in the media (2.5%, 5%, and 10%) and was lowest in flies maintained on control media versus flies maintained on creatine monohydrate treated media. The copulation data revealed that flies fed control media had a lower copulation duration than flies fed CrM supplemented media, and the mean copulation time rose as CrM concentration increased. Furthermore, fertility data demonstrated that flies fed control media produced a greater number of progeny than flies fed creatine monohydrate-treated media. With increasing CrM dosage, overall progeny production dropped (5% and 10%). As a result, the control mated faster, copulated shorter, and produced a greater number of progeny, whereas mating latency, copulation duration, and fertility of flies kept on 2.5% CrM enriched medium were not significantly different from the control. This means that 2.5% CrM supplementation in media had no influence on D. melanogaster reproductive fitness. Flies kept on 5% and 10% CrM supplemented medium had significantly reduced fertility but significantly higher mating latency and copulation length. It can be concluded that more 2.5% CrM supplementation on diet of D. melanogaster result in reduced reproductive fitness.
