Induction Of Callus and Shoot Proliferation Of A Medicinally Important Herb. CLEOME Rutidosperma Dc

Green globular callus was induced from leaf and node of Cleome rutidosperma DC On MS medium fortified with 2.0mg/ml BAP+1.0 IAA mg/ml maximum number of shoot buds were proliferated from the same explants on MS medium supplemented with 4.0mg/mlBAP+2.0mg/ml IAA. These plantlets were allowed for further growth on the same medium then transferred to full strength MS medium supplemented with 2mg/ml IBA for rooting. The rooted plantlets were transferred to peat + vermicompost pots for acclimatization