Research Article
DOI:
xxx-xxxx-xxx
Subject:
Medical
KeyWords:
Actinomycetes, soil sample, MRSA.
Abstract:
| The aim of the present study was to isolation and production of antibiotics from Actinomycetes spp. from the soil sample. The antibiotic produced by Actinomycetes spp. were tested against MRSA strains isolated from clinical sample. 20 soil samples were collected from different areas or localities of Nagpur city in different sterilized plastic locking bags. The soil samples were serially diluted on Actinomycete Isolation Agar (AIA) and different strains was identified on the basis of morphological, biochemical and cultural characteristics. The antibiotic susceptibility pattern was performed of well diffusion method. In this study, 28 Actinomycete isolates were obtained from 20 soil samples. The isolates were grown in starch casein broth and after incubation, broths were centrifuged and the secondary metabolites were extracted in ethyl acetate and used as test sample for |
antimicrobial assay against MRSA by agar well diffusion method in MHA medium. Among all the isolates, only 6 had shown the antagonistic effect against MRSA and the zones of inhibition obtained were in the range of 13-26mm. This study showed that the drug obtained from Actinomycetes, which is a group of organisms resembling both characteristics of bacteria and fungi showed antibacterial activity against MRSA.
