Development of microbial biofilms on the surfaces of manmade structures in the marine environment cause serious problems for the navies and for marine industries around the world. Marine microbes are promising potential sources of non toxic antifouling compounds as they can produce substances that inhibit not only the attachment of microorganism but also the settlement of invertebrates and macro algal spores. In order to evaluate the antifouling activity two bacterial strains were isolated from ship scrapping. Among that M5 bacterial strain showed remarkable antifouling activity. To investigate the antifouling secondary metabolite from M5 GC-MS analysis were performed and two compounds were identified such as Octadecanoic acid, methyl ester and 1R-(1à,3à,4á,5à)]-1-(Hydroxymethyl)-5-[(diisopropoxyphosphinyl)methyl]c. In addition M5 strain was subjected to morphological, physiological and genomic identification via, 16S rRNA gene sequencing and it was identified as Kytococcus sedentarius. This work suggested that Kytococcus sedentarius M5 could produce potent antifoulant compound which is responsible for antifouling activity.
