Isolation And Identification Of Microsporum Canis In 3 Lion's Cubsat Baghdad Zoo By Laboratory Tests And Pcr

Microsporum canis is a cosmopolitan zoophilic dermatophyte usually acquired from infected dogs or cats. Most prevalent in children, it has been implicated in infections of the scalp and skin and occasionally nails. In this study M. canis was recovered from 3 lion's cubs at Baghdad zoo (Al Zwraa Park) in March 2015. The fungal characterization was performed with classical mycological analysis by direct microscopic examination with 20% KOH and by fungal culture on Sabouraud's dextrose agar. The results confirmed by genotypical analysis using the ITS1 – ITS4 primer in PCR. DNA amplification of all isolates showed one distinct band with molecular weight of 740 bpamplified fragment on electrophoresis in agarose gel.