The objective of the present study was to isolate, characterize and identify the bacteriocin producing lactic acid bacteria from fish samples. Antimicrobial activity of the isolated strain was tested against spoilage pathogens. The extracted antimicrobial compound was also tested for some physiological conditions heat, pH and enzymes. The lactic acid bacteria strain was identified based on morphological, cultural, physiological and biochemical characters and carbohydrate utilization pattern identified as Lactobacillus plantarum. The extracted antimicrobial compound from the isolate exhibited no effect on the proteinase and pepsin. Although Trypsin has no effect on bacteriocin and active at 100°C. The effect of pH 2 to 12 on the crude protein extract was studied. The activity was not affected in pH 3 to 9.
