Sorghum is the fifth most important cereal crop in the world after wheat, rice, maize and barley. The grain had been used for consumption of both humans and livestock and also different genes of the plant serve many other important uses. The crop has been suffer from various types of diseases, majority of them are known to be caused by fungi, which are mostly seed borne. The present study aims to survey and isolate the seed borne fungi of sorghum in selected agroclimatic regions viz., Cuddalore, Dharmapuri, Salem and Villupuram, Tamil Nadu, India. The composite samples were collected from these regions carefully and transferred to the laboratory. And using PDA method, various fungal pathogens were isolated for the year 2016 and 2017 in the selected districts. It was observed that, species of Aspergillus, Mucor, Rhizopus occurrence is frequent in both the years along with Penicillium, Fusarium and Geotrichum. Presence of many pathogenic fungi with considerable number in the samples indicates the need of field surveys for these and other pathogens.