Transplantation is defined as the transfer (engraftment) of human cells, tissues or organs from a donor to recipient with an aim of restoring function(s) in the body. But in almost every country the number of willing organ donors falls short of the number of people on the waiting list by many thousands. As health care personnel we remain one of the most reliable sources for general population to get information about a relatively safe procedure. This study aims to determine the knowledge, attitude and practices towards organ donation amongst the Students & Staff members of Adhiparasakthi Dental College & Hospital, Melmaruvathur & Melmaruvathur Adhiparasakthi Institute of Medical Sciences & Research, Melmaruvathur and the variation amongst the study population and determination of the factors that cause the variation. The most significant factors which contributed to a positive attitude about organ donation were age, stream of study, father’s occupation, family’s monthly income, mother’s education level.