Silver nanoparticles are among the most commercialized inorganic nanoparticles due to their antimicrobial potential. In the present study the Lactobacillus species were isolated from cow milk and their probiotic properties were characterized. The formation of nanoparticles was first screened by measuring the absorbance peak at 430 nm using UV-vis spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The morphology of the synthesized AgNPs was determined using TEM, which indicated that the AgNPs were spherical in shape. The synthesized silver nanoparticles (AgNps) showed effective antibacterial activity against bacterial pathogens such as Eschirichia coli, Staphylococcus auerues, Pseudomonas aeruginosa, Proteus vulgaris, Salmonella typhimurium, Klebsiella pneumoniae and Shigella flexneri. The biosynthesized silver nanoparticles of Lactobacillus species showed a very strong inhibitory action against bacterial pathogens and the study supports that AgNPs can be used against gram-negative and gram-positive bacteria for the treatment of infectious diseases.
