management of root-rot complex disease and assessment of plant growth promoting characters in vegetable pea with native and commercial antagonistics through seed biopriming

Research Article
Devendra Singh Negi, Pradeep Kumar Sharma* and Rajan Kumar Gupta
DOI: 
xxx-xxxx-xxx
Subject: 
science
KeyWords: 
Trichoderma harzianum, Pseudomonas fluorescens, Trichoderma virens, Rhizoctonia solani, Fusarium solani, Biocontrol agents.
Abstract: 

This study was conducted to evaluate the seed treatment effects of native and commercial antagonistics for management of root rot complex disease and plant growth promoting activities in vegetable pea using individual and combination of bioagents. Initially, a survey was conducted in major vegetable growing areas in Uttarakhand, India to collect pathogen infected seedlings. Pathogenicity test showed that 50% samples were found infected with complex of Rhizoctonia solani and Fusarium solani pathogens, 33.0 % with Fusarium solani and 17.0% with Rhizoctonia solania. Field experiments were conducted in experimental area for investigating disease management and Plant growth activity. A marked increase in shoot, root and seedling lengths, vigor index, number of pods, number of grains per pods, pod weight and yield were observed in combination seed treatment effect of Trichoderma harzianum and Pseudomonas fluorescens. Among the all, antagonistic combinations of T.harzianum and P.fluorescens were found to be most effective in promoting the plant growth activities as well as increasing yield parameters of pea crop in field conditions. The observations revealed that T.harzianum and P.fluorescens combination are quite effective against root rot complex pathogen (% disease incidence (PDI); 11.9 %), followed by P. fluorescens (PDI; 15.4%) and Trichoderma harzianum + Pseudomonas fluorescens + Trichoderma virens (PDI; 15.9%) compared to untreated control (PDI; 21.6%). Disease severity (0-9 scale) was also observed and seed treatment with T.harzianum + P. fluorescens was found to have least disease severity (20.0 %) followed by T.harzianum+ P. fluorescens + T.virens(30 %), P. fluorescens(31.1 %).