The microbiological analysis of dirt particles obtained from the floors of Madonna University Teaching Hospital was investigated. The media used were nutrients agar for total aerobic plate count, MacConkey agar for coliforms count and Sabouraud dextrose agar for fungal count. The pour plate technique was employed. Colonial morphology, Gram staining and biochemical tests were used for identification and characterization. The analysis of variance (ANOVA) was used to show their significant difference. The mean total aerobic plate count ranged from 4.36 ± 0.47Log10cfu/g to 4.82 ± 0.69Log10cfu/g while the mean coliform count ranged from 3.81 ± 1.3Log10cfu/g to 4.42 ± 0.97Log10cfu/g. The fungal count ranged from 6.50 ± 0.60Log10cfu/g to 6.70 ± 0.18Log10cfu/g. The bacterial isolated were Pseudomonas aeruginosa, Staphylococcus aureus, Proteus mirabilis, Serratia marcescens, Escherichia coli and Klebsiella species. The fungi isolated were Rhizopus stolonifer, Mucor pusillus, Aspergillus flavus, Aspergillus niger, Trichophyton mentagrophyte, Alternaria species, Penicillum candidum, Microsporium gypseum. Cephalosprium species and Cladosporium species. The need for the determination of microorganisms present in hospital wards becomes necessary to serve as a guide for monitoring sanitation practices in the hospital wards.
