Many microorganisms seem to be involved in female reproductive failure in various ways. Infection of the lower genital tract seems to have little importance, if not, in the unlikely event of an occlusion. However, such infections, as well as those involving other parts of the male genito-urinary tract, may cause a microbial colonization. Virtually all parts of the female reproductive system may be influenced by infectious agents; some conditions, however, seem to have a greater impact on female fertility. Vaginal infections are of doubtful impact and cases of endometritis, leading to uterine synechiae, are less common than tubal occlusions resulting from salpingitis. Adhesions, caused by pelvic inflammatory disease, seem to affect the functional status of the tubes more harshly than that of the uterus. The aim of the present study was to carry out a microbiological investigation of endometrial samples of females suffering from unexplained infertility. Sample sizes of 168 women were evaluated by standard bacterial culture method and for fungal isolates. Bacterial infections were detected in 42.85% of infertility cases. Among the bacterial infections Enterobacter spp was the dominant followed by E.coli. The fungal infections were isolated in 65.47% of the infertility cases Candida spp were isolated from 5.36% of cases. Aspergillus was found only in 0.6% of cases. A combination of Candida and Aspergillus growth was found in a single case(0.6%). Following the isolation the organisms were sequenced then deposited in NCBI. The restriction sites and secondary structures were studied to confirm the emergence of new sub strains.
